Easy, Reliable Diagnostic Kit for Detection of Salmonella in Animal Feces

Description

All-In-One Tube Test Fecal Salmonella (2 Kits) Description/Intended Use:Animals become infected with Salmonella through their environment, by eating contaminated food, or from their mothers before they are even born or hatched. All-In-One Tube Fecal Salmonella is an inexpensive, easy-to-use and accurate detection kit for Salmonella in animal feces(pets, livestock and poultry) and their environments. A color change of solidculture medium from green to orange is considered positivePrinciple of the Test:All-In-One Tube Salmonella Stool Test containssterile two-phase culture media recommended by the FDA BacteriologicalAnalytical Manual (BAM)- Salmonella. The liquid medium is formulated with the modifiedsubstrates of BAM Media M132 (Rappaport-Vassiliadis broth) which support growthof Salmonella against high and low levels of competitive microflora in thestool sample. The solid medium is formulated with the chromogenic substrates whichincrease sensitivity and selectivity of the test.Benefits: · Easy-to-use withno preparation steps required · Color results areeasy to interpret – orange indicates presence of Salmonella spp. · Positive resultsin as little as 40-48 hours for stool and environmental samples · Self-containedtube eliminates risk of contamination · 3-month shelflife at refrigerated temperature (~4 ⁰C) Sensitivity: · DetectsSalmonella species down to 1-10 CFU Specificity: · Simultaneousculturing and detection in a single tube gives high selectivity in the presenceof other enteric bacteria and Gram-positive cells. Directions: 1. Fill All-In-One Tube (Figure 2) with 12 mL of MB2 blue. 2. Take out one cotton swab (Figure 4)with the clean hand and avoiding contact with the cotton tip. 3. Pre-moisten the cotton swab with neutralizingbuffer (NB) prior to sampling. 4. Collect a small amount of stool by inserting all the tip of the cotton swab into stool sample and rotate. 5. Insert the swab into the tube. 6. Screw the cap on tightly. 7. Incubate at roomtemperature (70-74 °F or 21-23 °C) for 40 - 48 hours. After incubation, invert the tube 3-4 times and then read the color just below tube line 2. Interpretation: Result is positive when the color just below tube line 2 turns orange. Green (-) Orange (+)